Transcriptional and translational analysis of the vaccinia virus late gene L65
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چکیده
منابع مشابه
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We prepared antiserum that reacted with a major core polypeptide of approximately 62,000 daltons (62K polypeptide), designated 4b, and its 74K precursor, designated P4b. A cell-free translation product of vaccinia virus late mRNA that comigrated with P4b was specifically immunoprecipitated. The late mRNA encoding P4b hybridized to restriction fragments derived from the left end of the HindIII A...
متن کاملThe vaccinia virus H5R gene encodes late gene transcription factor 4: purification, cloning, and overexpression.
The vaccinia virus late stage-specific transcription factor P3 was purified to homogeneity from HeLa cells that were infected in the presence of an inhibitor of viral DNA replication. The purified 36-kDa protein was digested with trypsin, and the peptides were analyzed by mass spectroscopy and amino-terminal sequencing. The purified factor was identified as the product of the vaccinia virus H5R...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1985
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.55.2.450-457.1985